Mutants of the N-3 R-Factor Conditionally Defective in hsp II Modification and Deoxyribonucleic Acid-Cytosine Methylase Activity
نویسندگان
چکیده
منابع مشابه
Biochemistry of Deoxyribonucleic Acid-defective Amber Mutants of Bacteriophage TP
Nucleotide pools were analyzed under different conditions of infection by bacteriophage T4, for three reasons: (a) as an approach to identifying the defective functions associated with conditional lethal phage mutants bearing uncharacterized lesions in DNA replication; (b) to gain information about the control of nucleotide synthesis in phage-infected Escherichia coli; and (c) as an aid to inte...
متن کاملProperties of bacteriophage T4 mutants defective in gene 30 (deoxyribonucleic acid ligase) and the rII gene.
In Escherichia coli K-12 strains infected with phage T4 which is defective in gene 30 [deoxyribonucleic acid (DNA) ligase] and in the rII gene (product unknown), near normal levels of DNA and viable phage were produced. Growth of such T4 ligase-rII double mutants was less efficient in E. coli B strains which show the "rapidlysis" phenotype of rII mutations. In pulse-chase experiments coupled wi...
متن کاملThe enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. IX. Deoxyribonucleic acid methylase in bacteriophage-infected Escherichia coli.
Infection of Escherichia coli B with bacteriophages Tl, T2, or T4 results in increase in the activity of deoxyribonucleic acid methylase assayed in crude extracts of the infected cells. The largest increase is observed after infection with T2. phage; T4 and Tl phages lead to smaller increases in that order of decreasing magnitude. After infection with T3, T5, or T6 phages, a decrease in activit...
متن کاملAmber mutants of bacteriophages T3 and T7 defective in phage-directed deoxyribonucleic acid synthesis.
Amber mutants of the related phages T3 and T7 were isolated and tested for their ability to restore-as the wild type does-thymidine incorporation in ultraviolet (UV)-irradiated, UV-sensitive, nonpermissive host bacteria (Escherichia coli B(s-1)). Most amber mutants had this ability. However, in both T3 and T7, mutants unable to promote thymidine incorporation under these conditions were found a...
متن کاملDeoxyribonucleic acid-deoxyribonucleic acid hybridization of R factors.
A new method of estimating deoxyribonucleic acid homology is presented and used to measure the homology between bacterial plasmids of different compatibility groups. Deoxyribonucleic acid of the P-group plasmid RP1, originally isolated in Pseudomonas aeruginosa, has considerable (50%) homology with an N-group plasmid of the Enterobacteriaceae.
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1974
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.120.1.234-239.1974